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Insights into the tumor microenvironment and human TRBV gene polymorphism revealed by long-amplicon immune repertoire sequencing

      Background: T cell receptor beta (TCRβ) immune repertoire analysis by next-generation sequencing is emerging as a valuable tool for research studies of the tumor microenvironment and potential immune responses to cancer immunotherapy. Here we describe an AmpliSeqTM-based TCRβ sequencing assay that leverages the long read capability of the Ion 530TM chip to provide coverage of all three complimentary determining region (CDR) domains of the human TCRβ chain. We demonstrate use of the assay to evaluate tumor-infiltrating T cell repertoire features, monitor manufacture of therapeutic T cells, and discover TRBV gene allele polymorphisms that may predict autoimmune disease or immune-mediated adverse events.
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