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Standard normoxic versus physiological hypoxic culture of AML patients' (pts) whole blood (WB) samples with immune modulatory kits yields comparable proportions of dendritic cells and functional results

      Background: For a clinical treatment strategy DCleu could be generated ex vivo and transferred to the pts or alternatively blasts modulated to leukemia-derived DC (DCleu) in vivo-resulting in Tcell activation: Combinations of GM-CSF+ PICIBANIL (I), or +PGE2 (K) or + PGE1 (M)are kits that convert blasts into a ‘DCleu-vaccine’ and induce antileukemic T-cell(memory) without induction of blast proliferation in a WB-culture-model, in the presence of soluble and cellular components of the pt. To simulate physiological conditions most we compared normoxic (N*) standard- (21%02) vs physiological hypoxic (H*) (10% O2) culture conditions.
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