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Background
Some hyperthermia studies in oncology deals with connected immune-effects. Modulated
electro-hyperthermia (oncothermia, Szasz et al. (2010) [
]) is an emerging curative treatment method. It is selectively focused in the malignant
cells without harming the non-malignant surrounding tissues Andocs et al. (2009) [
].
Aim
Our objective is studying the cell-death process caused by the treatment.
Method
HT29 human colorectal xenograft and C26 mouse colorectal allograft models were studied
in vivo, on in time-course investigations. We apply 13.56 MHz radiofrequency (RF) signal modulated by 1/f noise pattern. In silico models were
performed visualising the selection process. Samples were analysed by various histomorphological
and immunhistochemical analyses.
Results
A massive TUNEL positivity was produced. Up regulation of TRAILR2 (DR5), FAS and FADD
was observed. AIF nuclear translocalisation together with mitochondrial pore formation
and cytochrome-C release, as well as DNA fragmentation was measured. A damage associated
molecular pattern (DAMP) was formed, which are probably parts of the immunogenic cell-death
(ICD) of the selected malignant cells.
Conclusion
The DAMP associated, induced ICD can be a good basis for hyperthermia and immunotherapy
combination.
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References
- Oncothermia. Principles and Practices.Springer, 2010
- Electrom Biol Med. 2009; 28: 148-165
